Journal: mBio
Article Title: The Pseudomonas aeruginosa DedA protein PA4029 is an undecaprenyl phosphate flippase important for polymyxin resistance
doi: 10.1128/mbio.02408-25
Figure Lengend Snippet: Distribution of PA4029 orthologs or DUF368 proteins in Pseudomonas . ( a ) Venn diagram showing the distribution of orthologs of PA4029 or DUF368 domain-containing protein across 236 genomes from different Pseudomonas species and strains. Note that two strains ( P. stutzeri SLG510A3-8 and Pseudomonas resinovorans DSM 21078) apparently do not carry any PA4029 or DUF368 ortholog . ( b ) Fosmidomycin (FC) MIC of P. aeruginosa PAO1 (WT) and the ΔPA4029 mutant carrying the empty plasmid pME6032 (pME) or its derivative for ectopic expression of the DUF368 domain-containing protein CXK92_RS12370 of P. stutzeri KC (pMEDUF368). ( c ) Colistin (COL) MIC of the WT, the recombinant strain P rpsA::arn, and its ΔPA4029 mutant carrying the empty plasmid pME or its derivative pMEDUF368. Values in panels b and c are the mode of at least three independent experiments.
Article Snippet: To verify whether the DUF368 protein identified in some Pseudomonas species is involved in C55-P recycling, we ectopically expressed the ortholog of P. stutzeri KC (ATCC 55595) in P. aeruginosa cells lacking PA4029 and verified whether it can rescue the defects associated with impaired C55-P recycling.
Techniques: Mutagenesis, Plasmid Preparation, Expressing, Recombinant